Formation of three-dimensional fetal myocardial tissue cultures from rat for long-term cultivation
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چکیده
منابع مشابه
Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
A new procedure is introduced for the isolation of connective tissue fibers, called biomatrix, containing a significant portion of the extracellular matrix (basement membrane components and components of the ground substance). Biomatrix isolated from normal rat liver contains >90% of the tissue's collagens and all of the known collagen types, including types I and III and basement membrane coll...
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The possibility of cultivating N. gonorrhoeae on artificial media aids its isolation for diagnostic purposes and microbiological study. Numerous growing media and maintenance or transport media, have been described, each trying to meet the nutritive requirements of N. gonorrhoeae, which is a very fragile organism outside the human host. During recent years tissue cultures have been used as cult...
متن کاملTissue-culture light sheet fluorescence microscopy (TC-LSFM) allows long-term imaging of three-dimensional cell cultures under controlled conditions.
Fluorescence long-term imaging of cellular processes in three-dimensional cultures requires the control of media supply, temperature, and pH, as well as minimal photodamage. We describe a system based on a light sheet fluorescence microscope (LSFM), which is optimized for long-term, multi-position imaging of three-dimensional in-gel cell cultures. The system integrates a stable culture conditio...
متن کاملLumen formation in three-dimensional cultures of salivary acinar cells.
OBJECTIVE Development of an artificial salivary gland will benefit patients with xerostomia after radiation therapy for upper respiratory cancer. The goal is to devise a three-dimensional (3D) culture system in which salivary cells differentiate into polarized acini that express essential biomarkers and directionally secrete alpha-amylase. Differentiated acini-like structures in a 3D biomateria...
متن کاملThree-dimensional qualitative sonographic evaluation of fetal soft tissue.
Even though fetal growth restriction and macrosomia remain as major problems currently facing obstetricians, there is still no modality for the assessment of fetal soft tissue deposition and muscle mass in utero. A total of 52 fetuses from 29 to 41 weeks gestation were studied within 1 week before delivery using a transabdominal three-dimensional (3D) transducer (3.5 MHz). Their birth weights v...
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ژورنال
عنوان ژورنال: Developmental Dynamics
سال: 2006
ISSN: 1058-8388,1097-0177
DOI: 10.1002/dvdy.20871